ABSTRACT
OBJECTIVE@#To investigate whether kirenol, the major pharmacologically active compound of the Chinese medicinal herb , can protect mice from dextran sulfate sodium (DSS)-induced ulcerative colitis (UC).@*METHODS@#C57BL/6 mice with or without kirenol pretreatment were treated with DSS in drinking water for 7 days to induce UC. The symptoms of UC including weight loss, diarrhea and bloody stool were observed daily and graded using the disease activity index (DAI). Colon injury of the mice was assessed by measuring the length of the colon and HE staining of the colon tissue. The levels of inflammatory cytokines produced by the mesenteric lymph nodes (MLNs) lymphocytes were measured using enzyme-linked immunosorbent assay; the apoptosis of the lymphocytes and CD4 T cells was analyzed using flow cytometry.@*RESULTS@#The mice receiving pretreatment with kirenol showed obviously ameliorated symptoms of UC and milder pathological changes in the colon as compared with the control mice. Kirenol treatment significantly down-regulated the secretion of IFN-γ, IL-17A, IL-6 and TNF-α by the MLNs lymphocytes and increased the apoptosis of lymphocytes, especially CD4 T cells in the DSS-treated mice.@*CONCLUSIONS@#Kirenol can protect against T cell-mediated colon injury in DSS-treated mice possibly by suppressing the secretion of inflammatory mediators and inducing apoptosis of the inflammatory lymphocytes.
Subject(s)
Animals , Mice , Apoptosis , Colitis, Ulcerative , Cytokines , Dextran Sulfate , Diterpenes , Mice, Inbred C57BL , T-LymphocytesABSTRACT
Objective To investigate the value of serum 25 hydroxy vitamin D [25(OH)D] ,homocysteine (Hcy) and apolipoprotein B/A1 in patients with essential hypertension.Methods Totally 191 cases of essen-tial hypertension admitted in the hospital from May 2016 to May 2017 were selected as observation group ,and were divided into hypertension grade 1 group (54 cases) ,hypertension grade 2 group (78 cases) ,hypertension grade 3 group (59 cases) in accordance with the classification of hypertension.54 healthy people who under-went healthy assessment during the same period were selected as the control group.The changes of serum 25 (OH) D ,Hcy and apolipoprotein B/A1 ,and the sensitivity and the specificity of combined detection of 25 (OH)D ,Hcy and apolipoprotein B/A1 were compared.Results The serum 25 (OH) D level in the observa-tion group was lower than that in the control group ,while the levels of Hcy and apolipoprotein B/A1 were higher than those in the control group ,and the differences were statistically significant (P＜0.05).The level of serum 25 (OH) (OH) D in hypertension grade 3 group was lower than those in hypertension grade 2 group and hypertension grade 1 group ,but the levels of Hcy and apolipoprotein B/A1 were higher than those in hy-pertension grade 2 group and hypertension grade 1 group ,and the differencs were statistically significant (P＜0.05) ;the level of serum 25 (OH) D in hypertension grade 2 group was lower than that in hypertension grade 1 group ,but the levels of Hcy and apolipoprotein B/A1 were higher than those in hypertension grade 1 group , and the difference was statistically significant (P＜0.05).The sensitivity and the specificity of combined de- tection of 25 (OH) D ,Hcy and apolipoprotein B/A1 were higher than those of single detection of 25 (OH) D , Hcy and apolipoprotein B/A1.Conclusion Serum 25 (OH) D level decreased in patients with essential hyper-tension ,while Hcy and apolipoprotein B/A1 levels increased ,and the combined detection of 25(OH)D ,Hcy and apolipoprotein B/A1 had high sensitivity and specificity in diagnosis.